Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]

PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]

Uniprot : Q02242

Cat.No.:E-AB-F1131I
Applications: FCM
Isotype: Rat IgG2a, κ
Host: Rat

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Antigen Details

Alternate Names	PD-1, Programmed Death-1
Swissprot	Q02242
References	1. Nishimura H, et al. 2001. Science 291:319 2. Agata Y, et al. 1996. Int. Immunol. 8:765 3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706 4. Barber DL, et al. 2006. Nature 439:682 5. Keir ME, et al. 2005. J. Immunol. 175:7372 6. Koehn BH. et al. 2016.

Product Details

Form	Liquid
Clone No.	29F.1A12(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	PE/Cyanine5.5 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832I]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine5.5
	PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
Biotin Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
FITC Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
PE Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
APC Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More

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