Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]
Cat.No.:E-AB-F1131I
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Alternate Names | PD-1, Programmed Death-1 |
Swissprot | Q02242 |
References |
1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765 3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706 4. Barber DL, et al. 2006. Nature 439:682 5. Keir ME, et al. 2005. J. Immunol. 175:7372 6. Koehn BH. et al. 2016. |
Form | Liquid |
Clone No. | 29F.1A12(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | PE/Cyanine5.5 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832I] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE/Cyanine5.5 |
PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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