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PE/Cyanine5 Anti-Human HLA-A,B,C Antibody[W6/32]

Uniprot : P04439,P01889,P10321
  • Cat.No.:E-AB-F1130G

  • Applications: FCM
  • Isotype: Mouse IgG2a, κ
  • Host: Mouse

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Antigen Details

Alternate Names Major Histocompatibility Class I, MHC class I
Swissprot P04439,P01889,P10321
References
1. Barclay AN, et al. Eds. 1993. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.

Product Details

Form Liquid
Clone No. W6/32
Host Mouse
Isotype Mouse IgG2a, κ
Isotype Control PE/Cyanine5 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802G]
Reactivity Human
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine5
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells. They play an important role in cell-mediated immune responses and tumor surveillance.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

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