Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE/Elab Fluor® 594 Anti-Mouse CD162 Antibody[4RA10]
Cat.No.:E-AB-F1034P
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Alternate Names | P-selectin glycoprotein ligand 1,Selplg,PSGL-1,Selectin P ligand,CD162 |
Swissprot | Q62170 |
Gene ID | 20345 |
References |
1. Borges E, Eytner R, Moll T, et al.Blood. 1997; 90(5):1934-1942.
2. Borges E, Tietz W, Steegmaier M, et al. J Exp Med. 1997; 185(3):573-578. 3. Frenette PS, Denis CV, Weiss L, et al. . J Exp Med. 2000; 191(8):1413-1422. |
Form | Liquid |
Clone No. | 4RA10(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG1, κ |
Isotype Control | PE/Elab Fluor® 594 Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09822P] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE/Elab Fluor® 594 |
PE/Elab Fluor® 594 is designed to be excited by the blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 620 nm (e.g., a 610/20 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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