Alternate Names	B-cell receptor CD22,Cd22,B-lymphocyte cell adhesion molecule,BL-CAM,Sialic acid-binding Ig-like lectin 2,Siglec-2,T-cell surface antigen Leu-14,CD22,Lyb-8, Siglec2
Swissprot	P35329
Gene ID	12483
References	1. Symington FW et al. 1982. Immunogenetics 16:381. 2. Erickson LD et al. 1996. Int Immunol 8:1121. 3. Doody GM et al. 1995. Science 269:242.

Product Details

Form	Liquid
Clone No.	Cy34.1(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09793J]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PerCP/Cyanine5.5
	PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

The Cy34.1 monoclonal antibody specifically binds to the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g., A, BALB/c, CBA, C3H/He, C57BL, C57L, C58, SJL, SWR, but not AKR, DBA/1, DBA/2, NZB, PL). CD22 is expressed at high levels on mature peripheral B lymphocytes (follicular and marginal zone), B-1 cells (CD5+ B cells), and plasma cells. It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. Its intracellular domain is phosphorylated after cross-linking of antigen receptor or MHC class II antigen. It is involved in negative regulation of B-cell activation and protection from autoimmunity. B-cell proliferative responses to LPS or anti-mouse Ig µ chain are augmented in the presence of Cy34.1 mAb.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F10210	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021A	25μg, 100μg	Check More
Biotin Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021B	25μg, 100μg	Check More
APC Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021E	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021G	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021H	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021I	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD22 Antibody[Cy34.1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD22 Antibody[Cy34.1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD22 Antibody[Cy34.1]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021UE	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021UG	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021UH	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD22 Antibody[Cy34.1] E-AB-F1021UI	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD22 Antibody[Cy34.1]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD22 Antibody[Cy34.1]	25μg, 100μg	Check More

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