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PerCP/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2]

  • Cat.No.:E-AB-F1013UJ

  • Applications: FCM
  • Isotype: Rat IgG2b, κ
  • Host: Rat

To Purchase E-AB-F1013UJ

Size:
  • 25μg
  • 100μg
Price: $96
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Antigen Details

Alternate Names T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain,CD3E/D/G/Z,CD3e/d/g/z,CD3E/D/G/Z,CD3
Swissprot P04235,P11942,P22646,P24161
References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Product Details

Form Liquid
Clone No. 17A2
Host Rat
Isotype Rat IgG2b, κ
Isotype Control PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843J]
Reactivity Mouse
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PerCP/Cyanine5.5
PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse CD3 Antibody[17A2] 50μg, 500μg, 1mg Check More
Purified Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More
Biotin Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More
FITC Anti-Mouse CD3 Antibody[17A2] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse CD3 Antibody[17A2] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse CD3 Antibody[17A2] 50Tests, 100Tests, 100Tests×2 Check More
PerCP Anti-Mouse CD3 Antibody[17A2] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse CD3 Antibody[17A2] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD3 Antibody[17A2] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2] 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse CD3 Antibody[17A2] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse CD3 Antibody[17A2] 50Tests, 100Tests, 100Tests×2 Check More
PE/Elab Fluor® 594 Anti-Mouse CD3 Antibody[17A2] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Mouse CD3 Antibody[17A2] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Red 780 Anti-Mouse CD3 Antibody[17A2] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More
PE Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More
APC Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More
PerCP Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More
PE/Elab Fluor® 594 Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More
Elab Fluor® Violet 450 Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More
Elab Fluor® Red 780 Anti-Mouse CD3 Antibody[17A2] 25μg, 100μg Check More

Citations

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