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Phosphoenolpyruvate Carboxylase (PEPC) Activity Assay Kit

Cat.No.:E-BC-K613-M
Detection instrument: Microplate reader (320-360 nm, optimum wavelength: 340 nm)

Detection method: Colorimetric method

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48T

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Detection principle

Phosphoenolpyruvate carboxylase (PEPC) catalyzes phosphoenolpyruvate (PEP) to produce oxaloacetic acid, which further reacts with NADH. NADH has maximum absorption at 340 nm. The activity of PEPC can be calculated by measuring the change of absorbance value at 340 nm.

Performance characteristics

Synonyms	PEPC
Sample type	plant tissue
Sensitivity	0.72 U/L
Detection range	0.72-25.61 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Precision	Average inter-assay CV: 8.5-10.6%Average intra-assay CV: 1.5-4.3%
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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