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Pyruvate Decarboxylase (PDC) Activity Assay Kit

  • Cat.No.:E-BC-K500-M

  • Detection instrument: Microplate reader (330-350 nm, optimum wavelength: 340 nm)

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Detection principle

Pyruvate decarboxylase (PDC) mainly exists in yeast and is one of the key enzymes in ethanol fermentation. PDC catalyzes pyruvate decarboxylate to acetaldehyde, which reacts under the action of ethanol dehydrogenase (ADH), and catalyzes NADH to convert into NAD+. NADH has a characteristic absorption peak at 340 nm. The PDC activity can be calculated by measuring the OD value at 340 nm.

Performance characteristics

Synonyms PDC
Sample type serum, plasma, tissue and cell samples
Sensitivity 0.67 U/L
Detection range 0.67-27.73 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 15 min
Precision Average inter-assay CV: 3.100%Average intra-assay CV: 2.800%
Other instruments required Vortex mixer, Centrifuge
Other reagents required Normal saline (0.9% NaCl)
Storage -20℃
Valid period 12 months


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