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Rat REN(Renin) ELISA Kit

  • Cat.No.:E-EL-R0030

  • Reactivity: Rat

To Purchase E-EL-R0030

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat REN in samples. No significant cross-reactivity or interference between Rat REN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat REN concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat REN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat REN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat REN. You can calculate the concentration of Rat REN in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat REN were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat REN were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 52.60 150.60 411.70 56.30 160.50 427.40
Standard deviation 3.40 8.90 12.40 3.00 6.90 23.50
CV (%) 6.46 5.91 3.01 5.33 4.30 5.50

Recovery

The recovery of Rat REN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-99 94
EDTA plasma (n=8) 91-105 96
Cell culture media (n=8) 93-110 100

Linearity

Samples were spiked with high concentrations of Rat REN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsAngiotensinogenase
Research AreaCancer, Cardiovascular, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES (2021) IF: 4.222
    Effects of carotid baroreceptor stimulation on aortic remodeling in obese rats

    DOI: 10.1016/j.numecd.2021.01.021

    PMID: 33812737

    Sample: Tissue homogenate
  2. Oxidative Medicine and Cellular Longevity (2018) IF: 4.936
    Antihypertensive Effect of Ethanolic Extract from Acanthopanax sessiliflorus Fruits and Quality Control of Active Compounds

    DOI: 10.1155/2018/5158243

    Sample: serum
  3. EXPERIMENTAL BIOLOGY AND MEDICINE (2019) IF: 3.005
    Calcimimetic R568 improved cardiac remodeling by classic and novel renin-angiotensin system in spontaneously hypertensive rats:

    DOI: 10.1177/1535370219854325

    Sample: plasm
  4. LIFE SCIENCES (2020) IF: 3.647
    Taxifolin improves disorders of glucose metabolism and water-salt metabolism in kidney via PI3K/AKT signaling pathway in metabolic syndrome rats

    DOI: 10.1016/j.lfs.2020.118713

    PMID: 33157091

    Sample: Serum,Urine
  5. Journal of Translational Medicine (2016) IF: 3.694
    Modulation of the sympathetic nervous system by renal denervation prevents reduction of aortic distensibility in atherosclerosis prone ApoE-deficient rats

    DOI: 10.1186/s12967-016-0914-9

    Sample: Tissue homogenate
  6. MOLECULAR BIOLOGY REPORTS (2019) IF: 2.107
    var kunstleri in spontaneously hypertensive rats: possible involvement of renin–angiotensin–aldosterone system, endothelial function and anti-oxidant system

    DOI: 10.1007/s11033-019-04730-w

    Sample: serum,urine
  7. International Journal of Endocrinology (2019) IF: 2.287
    Aliskiren, Fosinopril, and Their Outcome on Renin-Angiotensin-Aldosterone System (RAAS) in Rats with Thyroid Dysfunction

    DOI: 10.1155/2019/5960563

    Sample: serum
  8. JOURNAL OF PHARMACY AND PHARMACOLOGY (2019) IF: 2.39
    The effect of eplerenone on the renin‐angiotensin‐aldosterone system of rats with thyroid dysfunction

    DOI: 10.1111/jphp.13168

    Sample: Serum
  9. Experimental and Therapeutic Medicine (2018) IF: 2.447
    Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin‑angiotensin system activity

    DOI: 10.3892/etm.2018.6734

    Sample: Plasma,Tissue homogenate
  10. Experimental and Therapeutic Medicine (2020) IF: 2.447
    Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin‑angiotensin system

    DOI: 10.3892/etm.2020.8759

    Sample: Cell lysate
  11. BMC Complementary and Alternative Medicine (2019) IF: 2.479
    Antihypertensive effects of the hydro-ethanol extract of Senecio serratuloides DC in rats

    DOI: 10.1186/s12906-019-2463-2

    PMID: 30819180

    Sample: Serum
  12. CHRONOBIOLOGY INTERNATIONAL (2019) IF: 2.562
    Valsartan chronotherapy reverts the non-dipper pattern and improves blood pressure control through mediation of circadian rhythms of the renin-angiotensin system in spontaneous hypertension rats

    DOI: 10.1080/07420528.2019.1610419

    Sample: plasma
  13. MOLECULES (2017) IF: 2.861
    Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS

    DOI: 10.3390/molecules22111956

    Sample: Cell culture supernatant
  14. Reviews in Cardiovascular Medicine (2021) IF: 2.93
    Histone deacetylase inhibitor, mocetinostat, regulates cardiac remodelling and renin-angiotensin system activity in rats with transverse aortic constriction-induced pressure overload cardiac hypertrophy.

    DOI: 10.31083/j.rcm2203113

    Sample: serum
  15. PHARMACEUTICAL BIOLOGY (2020) IF: 2.971
    Effect of phenylacetamide isolated from lepidium apetalum on myocardial injury in spontaneously hypertensive rats and its possible mechanism

    DOI: 10.1080/13880209.2020.1778043

    Sample: myocardial tissue
  16. CLINICAL AND EXPERIMENTAL HYPERTENSION (2019) IF: 1.522
    Standardized ethanol-water extract of Ficus deltoidea Angustifolia reduces blood pressure in spontaneously hypertensive rats

    DOI: 10.1080/10641963.2018.1506467

    PMID: 30648895

    Sample: Serum
  17. Chinese Herbal Medicines (2019)
    Effects and mechanism of total phenols of Magnolia officinalis combined with Maijunan Tablets on blood pressure of spontaneous hypertensive rats

    DOI: 10.1016/j.chmed.2018.12.004

    Sample: Plasma,Serum,Tissue homogenate
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