Recombinant 4E BP1 Monoclonal Antibody (E-AB-81520)
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, C6, CHO-K1 Verified Samples in IHC: Human breast cancer |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Hamster |
| Applications | WB, IHC-P |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human eIF4EBP1 |
| Abbre | 4E BP1 |
| Synonyms | 4E-BP1, 4EBP1, BP 1, Eif4ebp1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1, eIF4E binding protein 1, eIF4E-binding protein 1 |
| Swissprot | |
| Calculated MW | 13 kDa |
| Observed MW |
15 /20 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytosol |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Metabolism |
| Clone No. | R06-8E6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1),is a member of 4EBPs family,which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E,thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors,hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1,alpha,beta and gamma. |
Other Clones
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Other Formats
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Unconjugated
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