Recombinant Caveolin 1 Monoclonal Antibody (E-AB-81544)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela Verified Samples in IF: Human breast cancer, Hela |
| Dilution | WB 1:1000-1:2000, IHC 1:50-1:100, IF 1:20-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, IF |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant protein of human Caveolin-1 |
| Abbre | Caveolin 1 |
| Synonyms | 22 kD, BSCL3, CAV, CAV1, CGL3, Caveolin 1 caveolae protein 22kDa, Caveolin-1, Caveolin1, LCCNS, MSTP085, OTTHUMP00000025031, PPH3, VIP 21, VIP21, caveolae protein, caveolin 1 alpha isoform, caveolin 1 beta isoform, cell growth-inhibiting protein 32 |
| Swissprot | |
| Calculated MW | 20 kDa |
| Observed MW |
20 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Golgi apparatus membrane. Cell membrane. Membrane>caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Cardiovascular, Metabolism, Signal Transduction, Tags and Cell Markers |
| Clone No. | R05-6C3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Caveolin-1 (CAV1),a multifunctional protein,is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor . Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma . However,other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers,especially in oral cancer . Recent study also show that CAV1 is involved in astric Cancer . |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
