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Recombinant Human Brain Natriuretic Peptide/BNP Protein (His & Flag Tag)

Uniprot : P16860

Cat.No.:PKSH032127
Expression host: E.coli

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Description

Synonyms	Natriuretic peptides B;Gamma-brain natriuretic peptide;NPPB;BNP
Species	Human
Expression_host	E.coli
Sequence	His27-Arg102
Accession	P16860
Mol_Mass	11.0 kDa
AP_Mol_Mass	14 kDa
Tag	N-His-Flag

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the print
Reconstitution	Please refer to the printed manual for detailed information.

Background

Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNP precursor gives rise to N-terminal Pro BNP (NT-proBNP) and mature BNP. N-terminal proB-type natriuretic peptide (NT-proBNP); a useful marker of heart failure (HF); is considered to be secreted mainly from the ventricle; increased serum NT-proBNP levels are also encountered in conditions such as atrial fibrillation (AF) and atrial septal defect in patients without HF.

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