Synonyms	Interleukin-12 subunit alpha;IL-12 subunit p35;IL-12A;Cytotoxic Lymphocyte Maturation Factor 35 kDa
Species	Human
Expression_host	E.coli
Sequence	Arg 23-Phe 202
Accession	P29459
Mol_Mass	23.5 kDa
AP_Mol_Mass	28 kDa
Tag	C-His
Bio_Activity	Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBMC). The ED₅₀ for this effect is <49 pg/mL.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 0.1 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS,pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Citations

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