Recombinant Human VEGF165/VEGFA Protein (PKSH033475)
For research use only.
| Synonyms | MVCD1, VEGF, VEGF165, VPF, Vascular Endothelial Growth Factor Isoform 165 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ala27-Arg191 |
| Accession | P15692-4 |
| Calculated Molecular Weight | 19.1 kDa |
| Observed Molecular Weight | 18-24 kDa |
| Tag | None |
| Bio-activity | Immobilized Human VEGF165 at 2μg/ml (100 μl/well) can bind Human VEGF-A antibody. The ED50 of Human VEGF-A antibody is 2.20 ng/ml. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.01 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM Citrate, 8% Sucrose, 4% Mannitol, 0.05% Tween 80, pH4.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Human Vascular endothelial growth factor (VEGF); also known as VEGF-A and vascular permeability factor (VPF); belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms; of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling. |
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