RPMI-1640 is an improved Mccoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

General Information

Product Form	Liquid
Concentration	1×
pH	7.2-7.4
D-Glucose	2000mg/L
HEPES	Negative
L-glutamine	2mM
NaHCO₃	2000mg/L
Phenol red	Negative
Storage	2-8℃ ,Shading Light
Shipping	RT
Expiration date	24 months

Notes

1. This product is for research use only.;
2. This product is sterilized by 0.1μm filtration.;
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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