S100P Polyclonal Antibody
- +2
Price: $ 530
Price: $ 320
Price: $ 200
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB;IHC
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:SW480,HeLa,Mouse small intestine,Rat small intestine Verified Samples in IHC:Human colon carcinoma,Human placenta,Mouse testis |
Dilution |
WB 1:500-1:2000, IHC 1:100-1:200 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | A synthetic peptide of human S100P (NP_005971.1). |
Abbre | S100P |
Synonyms | S100P;MIG9 |
Swissprot | |
Calculated MW | 10kDa |
Observed MW |
13kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cytoplasm and Nucleus |
Concentration | 1mg/mL |
Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method | Affinity purification |
Research Areas | Cancer; Signal Transduction |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer. |