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Streptavidin(peroxidase/HRP conjugated)

  • Cat.No.:E-AB-1043

  • Applications: IHC,WB,ELISA

To Purchase E-AB-1043

Size:
  • 60μL
  • 120μL
  • 500μL
Price: $21
Qty:

Antigen Information

Alternate Names Streptavidin

Product Information

Conjugation HRP
Purification Method Affinity purification
Concentration 0.5 mg/mL
Storage Buffer PBS with 50% glycerol, PH7.4
Recommended dilution
IHC-F 1:100-500
IHC-P 1:100-200
WB 1:3000-10000(Enhanced chemiluminescent detection)
WB 1:500-3000(DAB detection)
Direct ELISA 1:10000-100000(TMB detection)

Shipping&Storage

Shipping Biological ice pack at 4 ℃
Stability & Storage Store at -20℃. Avoid freeze / thaw cycles.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.

Citations

  1. MOLECULAR BIOLOGY REPORTS (2021) IF: 2.316
    The investigation of glutamate transporter 1 (GLT-1) degradation pathway in glioblastoma cells

    DOI: 10.1007/s11033-021-06407-9

    PMID: 34003424

    Sample: Cell Sample
  2. BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743
    Ricolinostat (ACY-1215) inhibits VEGF expression via PI3K/AKT pathway and promotes apoptosis in osteoarthritic osteoblasts

    DOI: 10.1016/j.biopha.2019.109357

    Sample: Oa Osteoblasts
  3. ANALYTICA CHIMICA ACTA (2022) IF: 6.911
    Investigating immunosensor for determination of depression marker-Apo-A4 based on patterning AuNPs and N-Gr nanomaterials onto ITO-PET flexible electrodes with amplifying signal

    DOI: 10.1016/j.aca.2022.340217

    PMID: 35998995

    Sample: Ab-Aunps@N-Gr@
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Reviews/Q&A

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  • Reviews
  • Q&A
Q

D******dSubmitted [ Dec 30 2023 ]

Asked: I\'m using this kit to detect intraepidermal nerve fiber density in formalin-fixed paraffin sectioned skin biopsy, I get the nerves but heavy background at the keratinocytes due to endogenous biotin non-specific staining, other kits get reliable results than this. So, what am I doing wrong? what is the appropriate concentration and application time? I used 1:200 up to 1:400 and incubation time 30 to 60 minutes, nearly the same results?

Reply

A

adminSubmitted [ Dec 30 2023 ]

Answered: Hi Dr.Ahmed, For the indicators you did, could you please provide the specific samples as well as the experimental conditions and result graphs? Could you also provide the item numbers of the other kits you used? Is it also from Elabscience?

Q

A************* Submitted [ Dec 29 2023 ]

Asked: What is the optimal duration for applying? I get a huge nonspecific keratinocytes staining that I suppose to be related to it?

Reply

A

adminSubmitted [ Dec 29 2023 ]

Answered: Incubation at 37°C for 30 minutes is usually sufficient

A

adminSubmitted [ Dec 29 2023 ]

Answered: Hi Ahmed, for the indicators you did, could you please provide the specific samples as well as the experimental conditions and result graphs? Could you also provide the item numbers of the other kits you used? Is it also from Elabscience?

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