Tau Polyclonal Antibody (E-AB-70075)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse brain, Rat brain Verified Samples in IHC: Human brain, Rat brain |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:500 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Tau |
| Abbre | Tau |
| Synonyms | AI413597, AW045860, DDPAC, FLJ31424, FTDP 17, G protein beta1/gamma2 subunit interacting factor 1, M, MAPT, MAPTL, MGC134287, MGC138549, MGC156663, MSTD, Microtubule associated protein tau, Microtubule associated protein tau isoform 4, Microtubule-associated protein tau |
| Swissprot | |
| Calculated MW | 40-70 kDa |
| Observed MW |
40-70 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm>cytosol. Cell membrane. Cytoplasm>cytoskeleton. Cell projection>axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components. |
| Concentration | 200 μg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Neuroscience, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. |
Other Clones
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Unconjugated
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