Introduction
Triton X-100 (Polyethylene Glycol
Octyl Phenyl Ether) is a non-ionic surfactant (or detergent). The molecular
weight is 646.86 (C34H62O11). It can dissolve lipid to increase the permeability of cell membrane to
antibody.
The concentration of Triton
X-100 in immunocytochemistry is 1% or 0.3%, in which 1% Triton X-100 is usually
used for rinsing tissue samples, 0.3% Triton X-100 is usually used for diluting
serum, 0.1% Triton X-100 is commonly used for lamp (loop mediated isothermal
amplification), preparation of BSA, etc.
Components
|
Cat
|
Products
|
10 mL
|
50 mL
|
100 mL
|
Storage
|
|
E-IR-R122
|
Triton X-100
|
10 mL
|
50 mL
|
100 mL
|
RT
|
Instructions
This product is 100% Triton X-100
solution. It is recommended to prepare 30% Triton X-100 stock solution, and
dilute to the required concentration when it is needed.
Preparation of 30% Triton X-100 stock
solution: Take 3 ml of Triton X-100 and add it to 7 ml of 1× PBS buffer, the mixture is 30% Triton
X-100.
Dilute the 30% Triton X-100 stock solution
with 1× PBS buffer to
the required concentration before use.
Storage
Store at room temperature for 12 months. Avoid of
freezing.
.
Cautions
1. This product is for research
use only. It couldn’t be used for clinical diagnosis or treatment, food or
medicine, and can’t be stored in residence.
2. For your safety and health,
please wear the lab coat and disposable gloves before the experiments.
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