VH/VB7(Biotin) ELISA Kit

Biotin microtiter plate test is a microbiological method for the quantitative determination of total biotin (added and natural biotin) in food and pharmaceutical products. The microbiological test system is in accordance with AOAC international norms.

Biotin is extracted from the sample and the extract is diluted. The biotin assay-medium and the diluted extract are pipetted into the wells of a microtiter plate which is coated with Lactobacillus plantarum. The growth of Lactobacillus plantarum is dependent on the supply of biotin. Following the

addition of biotin as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 - 48 h.

The intensity of metabolism or growth of Lactobacillus plantarum in relation to the extracted biotin is measured as turbidity and compared to a standard curve. The measurement is done using a microtiter plate reader at 610 - 630 nm (alternatively at 540 - 550 nm).