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Vitamin E (VE) Colorimetric Assay Kit

  • Cat.No.:E-BC-K033-M

  • Detection instrument: Microplate reader(525-533 nm,optimum wavelength: 533 nm)

To Purchase E-BC-K033-M

Size:
  • 96T
  • 48T
Price: $200
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Detection principle

Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. After colorimetric assay, VE content can be figured out according to the standard curve or calculated through formula.

Performance characteristics

Synonyms VE
Sample type Serum,plasma,tissue
Sensitivity 0.95 μg/mL
Detection range 0.95-40 μg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 30 min
Precision Average inter-assay CV: 6.3%Average intra-assay CV: 3.9%
Other instruments required Micropipettor, Centrifuge, Vortex mixer
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol, N-heptane
Storage 2-8℃
Valid period 6 months

Images

The level of vitamin E was significantly lower (*P<0.05) in arsenic treated group and increased in arsenic + melatonin group (#P<0.05) comparing to control.

N Abdollahzade et al found that melatonin attenuated oxidative DNA damage in rats and attenuate chronic arsenic neurotoxicity. Vitamin E level of rat brain tissue was determined using VE colorimetric assay kit (E-BC-K033-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.95-40 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Mouse serum

1

Chicken serum

1

10% Mouse liver tissue homogenate

1

10% Mouse brain tissue homogenate

1

10% Rat kidney tissue homogenate

1

10% Rat lung tissue homogenate

1

10% Rat spleen tissue homogenate

1

10% Rat heart tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Ralated product

Cat Number Product Name
E-BC-K034-M Vitamin C (VC) Colorimetric Assay Kit

Citations

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