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Vitamin E (VE) Colorimetric Assay Kit

Cat.No.:E-BC-K033-M
Detection instrument: Microplate reader(525-533 nm,optimum wavelength: 533 nm)

Detection method: Colorimetric method

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48T

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Protocols for metabolism assays

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Detection principle

Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. After colorimetric assay, VE content can be figured out according to the standard curve or calculated through formula.

Performance characteristics

Synonyms	VE
Sample type	Serum,plasma,tissue
Sensitivity	0.95 μg/mL
Detection range	0.95-40 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 6.300%Average intra-assay CV: 3.900%
Other instruments required	Micropipettor, Centrifuge, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol, N-heptane
Storage	2-8℃
Valid period	12 months

Images

The level of vitamin E was significantly lower (*P<0.05) in arsenic treated group and increased in arsenic + melatonin group (#P<0.05) comparing to control.

N Abdollahzade et al found that melatonin attenuated oxidative DNA damage in rats and attenuate chronic arsenic neurotoxicity. Vitamin E level of rat brain tissue was determined using VE colorimetric assay kit (E-BC-K033-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.95-40 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1
Chicken serum	1
10% Mouse liver tissue homogenate	1
10% Mouse brain tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat lung tissue homogenate	1
10% Rat spleen tissue homogenate	1
10% Rat heart tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Nutrients (2023) IF: 6.706
Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets

DOI: 10.3390/nu15040832

Sample: serum

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