VPS35 Polyclonal Antibody (E-AB-53569)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse brain, Human cerebella, A549 Verified Samples in IHC: Human brain, Human lung cancer |
| Dilution | WB 1:500-1:2000, IHC 1:20-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human VPS35 |
| Abbre | VPS35 |
| Synonyms | DKFZp434E1211, DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, MEM 3, MEM3, Maternal embryonic 3, Maternal-embryonic 3, PARK17, TCCCTA00141, Vacuolar protein sort, Vacuolar protein sorting 35, Vacuolar protein sorting 35 (yeast), Vacuolar protein sorting 35 homolog, hVPS35 |
| Swissprot | |
| Calculated MW | 92 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm. Membrane. |
| Concentration | 0.4 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene belongs to a group of vacuolar protein sorting (VPS) genes.The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network.The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function.Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. |
Other Clones
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Unconjugated
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