Caspase 1 Activity Assay Kit(Colorimetric Method) (E-CK-A381)
For research use only.
| Detection Principle | Caspase 1, also known as Interkeukin 1β Conberting Enzyme (ICE), is the only caspase in the caspase family that can cleave IL-1β prefixes or IL-18 prefabrication. Caspase 11 can cleave 45 kD caspase 1 preparation to produce 20 kD and 10 kD fragments, which can form heterodimer and further form tetramer by two heterodimers. Caspase 1 regulates apoptosis by cleaving Bcl-XL and the associated immune response by cleaving cytokine precursors, or regulates pyroptosis by hydrolyzing Gastermin D (GSDMD). This caspase 1 activity assay kit is based on caspase 1 that can catalyze the substrate AC-YVAD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 1 can be calculated by determining the absorbance at 405 nm. |
| Detection Method | Colorimetric method |
| Sample Type | Cell and tissue samples |
| Assay Time | 4-6 hours |
| Detection Instrument | Spectrophotometer |
| Other Reagents Required | PBS(E-BC-R187), Bradford Protein Determination Kit(E-BC-K168-S or E-BC-K168-M) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Expiration date | 6 months |
| Shipping | Ice bag |
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