Fetuin B Polyclonal Antibody(Capture/Detector) (AN003760P)
For research use only.
| Verified Samples |
Verified Samples in ELISA: Recombinant Human Fetuin B protein, Human serum, Human plasma Verified Samples in WB: Human plasma |
| Dilution | ELISA Capture 2-8 μg/mL, ELISA Detector 0.1-0.4 μg/mL, WB 1:500-1:1000 |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | ELISA Capture/Detector, WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human Fetuin B Protein expressed by Mammalian |
| Abbre | Fetuin B |
| Synonyms | 16G, IRL, 16G2, 2310011O17Rik, AI255764, D17980, Fet, Fetuin beta, Fetuin-B, Fetuin-like protein, Fetuin-like protein IRL685, Gugu, IRL685, Pp63, FETUB |
| Swissprot | |
| Calculated MW | 42 kDa |
| Observed MW |
45 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300. |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cardiovascular, Neuroscience |
| Conjugation | Unconjugated |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Fetuins are members of the cystatin superfamily of cysteine protease inhibitors. Additional members of this superfamily are kininogen and histidine-rich glycoprotein. Fetuin A and B are two known members of the fetuin family. Hepatocytes are believed to be the principal cellular source, but other cell types also express it. Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is an inhibitor of basic calcium phosphate precipitation and a negative acute-phase protein. Normal circulating levels of Fetuin A in adults (300‑600 ug/mL) fall significantly (30‑50%) during injury and infection. Fetuin B is a newer member whose function is not fully characterized. Fetuin A and B display similarities and differences in their characteristics. Fetuin B exhibits reduction of calcification, while both mRNA levels were down‑regulated during the acute phase in inflammation-induced rats. However, they share only 20% amino acid sequence identity. The amounts of Fetuin B in human serum, unlike Fetuin A, vary with gender and are higher in females than in males. |
Other Clones
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Unconjugated
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