Glycolate Oxidase Activity Assay Kit (E-BC-K692-S)

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All	Size	Price	Qty
	100Assays	$ 350.00
	50Assays	$ 220.00

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Detection Principle	Glycolate oxidase catalyzes sodium glycolate substrate to form glyoxylic acid, which reacts with phenylhydrazine hydrochloride to form phenylhydrazone glyoxalate. The substance has an absorption peak at 324 nm, and its OD value is proportional to the concentration of phenylhydrazone glyoxalate in a certain range, and the amount of phenylhydrazone generated reflects the activity of glycolate oxidase.
Sample Type	Plant tissue
Detection Method	Colorimetric method
Detection Instrument	Spectrophotometer (324 nm)
Research Area	Plant Physiology Series
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.3 U/mL
Detection Range	0.3-350 U/mL
Precision	inter-assay CV: 8.2% \| intra-assay CV: 1.1%
Sample Volume	50 μL(tissue homogenate)
Assay Time	30 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Oxalis corniculata tissue homogenate	1
10% Ginkgo biloba tissue homogenate	1
10% Cactus tissue homogenate	1
10% Bamboo leaf tissue homogenate	1
10% Osmanthus fragrans leaf tissue homogenate	4-8
10% Camphor trees leaf tissue homogenate	3-5

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.