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Mouse NOV (Nephroblastoma Overexpressed Gene) ELISA Kit (E-EL-M3115)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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All Size Price Qty
96T $ 495.00
48T $ 396.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 74.32 pg/mL
Detection Range 125-8000 pg/mL
Sample Volume 100 μL
Manual Operation Time 1 h
Total Assay Time 3 h 30 min
Reacitivity Mouse
Specificity This kit recognizes NOV in samples.No significant cross-reactivity or interference between NOV and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NOV . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NOV and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NOV , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of NOV . You can calculate the concentration of NOV in the samples by comparing the OD of the samples to the standard curve.
NOV (CCN3) is a member of the CCN family of secreted,cysteine-rich regulatory proteins that share four conserved modules which confer distinct biological activities. These modules include an insulin-like growth factor binding domain,a von Willebrand type C domain,thrombospondin-1 domain,and a C-terminal cysteine knot domain. It is widely expressed developmentally,especially in the muscle,endothelium,nervous system,adrenal cortex,and chondrocytes. NOV (CCN3) acts through the Notch and BMP signaling pathways to modulate self-renewal and maturation of a number of cell lineages including hematopoietic,osteogenic,and chondrogenic lineages. NOV (CCN3) is also a putative ligand for integrin receptors and is tightly associated with the extracellular matrix. Its interaction with integrins alpha v beta 3 and alpha 5 beta 1 mediates endothelial cell adhesion induces chemotaxis and promotes angiogenesis. The elevated expression of NOV (CCN3) is associated with certain tumors such as Wilm’s tumor and most nephroblastomas.
Gene Alias Ccn3
Gene ID 18133
Uniport ID Q64299
Protein Alias Ccn3
Research Area Cell Biology
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