Oct-1/2 Monoclonal Antibody (E-AB-22047)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela, Jurkat, HepG2 Verified Samples in IHC: Human breast cancer Verified Samples in IF: Human breast cancer |
| Dilution | WB 1:500-2000, IHC 1:50-300, IF 1:100-1:300 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB, IHC-p, IF |
| Clonality | Monoclonal |
| Immunogen | Synthetic Peptide |
| Abbre | Oct1 |
| Synonyms | Lymphoid-restricted im, NF-A1, OCT1, OCT2, OTF-1, OTF1, OTF2, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1, POU domain, POU2F1, POU2F2, class 2, transcription factor 1, transcription factor 2 |
| Swissprot | |
| Calculated MW | 76 kDa |
| Observed MW |
89 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endoplasmic reticulum, endoplasmic reticulum, Nucleus, nucleoplasm, nucleus, RNA polymerase II transcription factor complex, Other locations: intracellular membrane-bounded organelle. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Protein A purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling |
| Clone No. | 5G3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The OCT1 transcription factor was among the first identified members of the POU transcription factor family. Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT. |
Other Clones
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Other Formats
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Unconjugated
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