PERK Polyclonal Antibody (E-AB-70366)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, 293T, Mouse lung, Mouse skin, Mouse brain |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant protein corresponding to Mouse PERK |
| Abbre | PERK |
| Synonyms | DKFZp781H1925, E2AK3, EC 2.7.11.1, Eif2ak3, Eukaryotic translation initiation factor 2 alpha kinase 3, Eukaryotic translation initiation factor 2-alpha kinase 3, HRI, Heme regulated EIF2 alpha kinase, HsPEK, Pancreatic eIF2 alpha kinase, Pancreatic eIF2-alpha kinas |
| Swissprot | |
| Calculated MW | 140 kDa |
| Observed MW |
140 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endoplasmic reticulum membrane. |
| Concentration | 100 μg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Metabolism |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis.It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins.Mutations in this gene are associated with Wolcott-Rallison syndrome. |
Other Clones
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Unconjugated
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