Pg(Progesterone) ELISA Kit

Size	Price	Qty
96T	$ 495.00
48T	$ 396.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Sensitivity	0.15 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	50 μL
Total Assay Time	2 h 30 min
Reacitivity	Universal
Specificity	This kit recognizes Universal Pg in samples.No significant cross-reactivity or interference between Universal Pg and analogues was observed
Recovery	80%-120%
Sample Type	Serum, Plasma, Urine, Saliva
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal Pg. During the reaction, Universal Pg in the sample or standard competes with a fixed amount of Universal Pg on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal Pg. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal Pg in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Background

Progesterone (progesterone), also known as progesterone, progesterone, is the main progesterone secreted by the ovary with biological activity. Its chemical formula is C21H30O2, the main role is to protect the female endometrium, during female pregnancy, progesterone hormone can provide support and protection for the early growth and development of the fetus, and can play a certain calming effect on the uterus.

Research Area

Signal Transduction

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Pg(Progesterone) ELISA Kit (E-EL-0154)

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