Recombinant Chromogranin A/CHGA/CGA Monoclonal Antibody (AN300073P)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, SH-SY5Y Verified Samples in IF: HepG2 |
| Dilution | WB 1:500-1:1000, ICC/IF 1:20-1:100, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, ICC/IF |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the human Chromogranin A/CHGA/CGA |
| Abbre | CHGA |
| Synonyms | CHGA, CGA, Chromogranin-A, Pituitary secretory protein I, SP-I, Chromogranin A |
| Swissprot | |
| Calculated MW | 50 kDa |
| Observed MW |
50 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted, Cytoplasmic vesicle. |
| Tissue Specificity | Detected in cerebrospinal fluid (at protein level). Detected in urine (at protein level). |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Tags & Cell Markers, Signal Transduction, Cancer |
| Clone No. | 11D9 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Human Chromogranin A (CHGA) an acidic protein and a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells such as islet beta cell secretory granules in pancreas. Chromogranin A expression has been oberserved in neuroendocrine tumors, medullary thyroid carcinoma, as well as carcinoid tumors. |
Other Clones
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Other Formats
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Unconjugated
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