Recombinant HPGD/15-PGDH Monoclonal Antibody (AN300531P)
For research use only.
| Verified Samples |
Verified Samples in WB:?LOVO Verified Samples in IP: Caco-2 |
| Dilution | WB 1:500-1:2000, IP 1-4 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Mouse |
| Applications | WB, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Mouse HPGD/15-PGDH protein |
| Abbre | HPGD |
| Synonyms | Pgdh, 15-PGDH, AV026552, Pgdh1, Hpgd, 15 hydroxyprostaglandin dehydrogenase [NAD+], 15 PGDH, 15-hydroxyprostaglandin dehydrogenase [NAD+], 15PGDH, Hydroxyprostaglandin dehydrogenase 15 (NAD), NAD+ dependent 15 hydroxyprostaglandin dehydrogenase, OTTHUMP00000218960, OTTHUMP00000219016, OTTHUMP00000219018, PHOAR1, Prostaglandin dehydrogenase 1, SDR36C1, Short chain dehydrogenase/reductase family 36C member 1 |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW |
27 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Tissue Specificity | Expressed in proximal convoluted tubules of the kidney, where it colocalizes with the prostaglandin transporter SLC22A22 (at protein level) |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Clone No. | 7A7 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | 15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone, and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoarthropathy autosomal recessive (PHOAR), cranio-osteoarthropathy (COA), and isolated congenital nail clubbing. |
Other Clones
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Unconjugated
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