Recombinant Human 4-1BB/TNFRSF9 Protein (Fc & His Tag) (PKSH032027)
For research use only.
| Synonyms | 4-1BB ligand receptor, CD137, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Leu24-Gln186 |
| Accession | Q07011 |
| Calculated Molecular Weight | 44.0 kDa |
| Observed Molecular Weight | 55-75 kDa |
| Tag | C-Fc-His |
| Bio-activity | Immobilized Human 4-1BB-Fc-His at 2μg/ml (100 μl/well)can bind Anti-Human CD137 mAb .The ED50 of Anti-Human CD137 mAb is 0.41ug/ml. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Tumor necrosis factor receptor superfamily member 9(TNFRSF9) is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is a single-pass type I membrane protein which contains 4 TNFR-Cys repeats. The human and mouse proteins share 60% amino acid sequence identity. It is absent from naive T cells; but upregulated and continually expressed following T cell activation. It is a receptor for TNFSF9/4-1BBL; and possibly active during T cell activation. |
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