Recombinant NCR3/CD337 Monoclonal Antibody (AN300339P)
For research use only.
| Verified Samples | Verified Samples in WB:?K562 |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human NCR3/CD337 Protein |
| Abbre | NCR3 |
| Synonyms | NCR, NKp, Activating Natural Killer Receptor p, Natural Cytotoxicity Triggering Receptor, NK-p, DAAP-90L, NCR3, 1C7, CD337, LY117, MALS, NKp30, Activating Natural Killer Receptor p30, DAAP-90L16.3, Natural Cytotoxicity Triggering Receptor 3, Natural Killer Cell p30-Related Protein, NK-p30, NCR3, Activating NK A1 receptor, CD337 antigen, Lymphocyte antigen 117, Natural killer cell p30 related protein, NCTR3 |
| Swissprot | |
| Calculated MW | 22 kDa |
| Observed MW |
14 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Selectively expressed by all resting and activated NK cells and weakly expressed in spleen. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Stem Cells |
| Clone No. | 6G8 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Unconjugated
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