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100μL $ 380.00
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For research use only.

Verified Samples Verified Samples in WB: HELA, 293T, MCF7
Verified Samples in FCM: Hela
Verified Samples in IF: MCF7
Dilution WB 1:500-1:2000,  FCM 1:100-1:500,  ICC/IF 1:100-1:500,  IP 1-4 μL/mg of lysate
Isotype IgG
Host Rabbit
Reactivity Human
Applications WB,  FCM,  ICC/IF,  IP
Clonality Rabbit Monoclonal
Immunogen Recombinant Human STUB1 protein
Abbre STUB1
Synonyms STUB,  SCAR,  UBOX,  Antigen NY-CO,  CLL-Associated Antigen KW,  STIP1 Homology and U Box-Containing Protein,  NY-CO,  HSPABP,  SDCCAG,  STUB1,  CHIP,  HSPABP2,  NY-CO-7,  SCAR16,  SDCCAG7,  UBOX1,  Antigen NY-CO-7,  Carboxy Terminus of Hsp70-Interacting Protein,  CLL-Associated Antigen KW-8,  E3 Ubiquitin-Protein Ligase CHIP,  STIP1 Homology and U Box-Containing Protein 1,  PP1131,  STIP1 homology and U-box containing protein 1
Swissprot
Calculated MW 35 kDa
Observed MW 36 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Concentration 1 mg/mL
Buffer 0.2 μm filtered solution in PBS
Purification Method Protein A
Research Areas Signal Transduction,  Epigenetics and Nuclear Signaling
Clone No. 9D2
Conjugation Unconjugated
Storage This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Shipping Ice bag
background This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2.
Other Clones

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Unconjugated

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