Recombinant TNF-alpha Monoclonal Antibody (AN300581P)
For research use only.
| Verified Samples | Verified Samples in IF: Rat spleen |
| Dilution | ICC/IF 1:20-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Rat |
| Applications | ICC/IF |
| Clonality | Monoclonal |
| Immunogen | Recombinant Rat TNF-alpha Protein |
| Abbre | TNF |
| Synonyms | MGC, Tnfsf, TNF-a, Cachectin, DIF, ICD1, ICD2, Membrane Form, MGC124630, N-terminal fragment, NTF, RATTNF, Soluble Form, TNF-alpha, Tumor Necrosis Factor, Tumor Necrosis Factor Ligand Superfamily Member 2, Tnf, Tnfa, Tnfsf2 |
| Swissprot | |
| Cellular Localization | Secreted, Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Clone No. | B516 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. |
Other Clones
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Other Formats
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Unconjugated
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