Sialic Acid (SA) Colorimetric Assay Kit (Enzyme Method) (E-BC-K813-M)
For research use only.
| Detection Principle |
Sialic acid (SA) is widely present in animals and microorganisms, usually in the form of oligosaccharides, glycolipids or glycoproteins, and is an important component of cell membranes. SA is located at the ends of cell membrane glycoproteins and glycolipids, and participates in various physiological functions such as cell recognition, adhesion and contact inhibition. It is a broad-spectrum tumor marker. When normal cells transform into malignant tumor cells, the structure and content of glycoproteins and glycolipids on the cell surface undergo significant changes. SA falls off and enters the blood, increasing the content of SA in serum. Measuring its level is of great significance for the auxiliary diagnosis, therapeutic effect observation and prognosis judgment of malignant tumors. SA is converted into pyruvic acid under the action of a series of enzymes. Pyruvic acid is converted into lactic acid and NAD+ under the action of lactate dehydrogenase and NADH. The concentration of SA in the sample can be indirectly measured by measuring the decrease in the absorbance of NADH at 340 nm. |
| Sample Type | Serum,Plasma,Saliva,Milk |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Metabolic Diseases |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.05 mmol/L |
| Detection Range | 0.05-4.00 mmol/L |
| Precision | inter-assay CV: 6.3-7.3% | intra-assay CV: 3.2-4.5% |
| Sample Volume | 7 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum (plasma) | 1 |
| Mouse serum (plasma) | 1 |
| Rat serum (plasma) | 1 |
| Human milk | 1 |
| Human saliva | 1 |
| Rabbit serum | 1 |
| Chicken serum | 1 |
| Porcine serum | 1 |
| Horse serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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