Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit (E-BC-F032)

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All	Size	Price	Qty
	96T	$ 400.00
	48T	$ 280.00

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Detection Principle	Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.
Sample Type	Serum,Plasma,Animal tissue,Cell
Detection Method	Fluorometric method
Detection Instrument	Fluorescence microplate reader (Ex/Em=535 nm/590 nm)
Research Area	Glycolysis And Lipid Metabolism
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.12 μmol/L
Detection Range	0.12-30 μmol/L
Precision	inter-assay CV: 7.3% \| intra-assay CV: 1.7%
Sample Volume	50 μL
Assay Time	20 min

Cat.No.	Product Name	Sizes
E-BC-K004-M	Free Cholesterol (FC) Colorimetric Assay Kit	500Assays , 96T , 48T
E-BC-K109-M	Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)	500Assays , 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	100-300
Rat serum	100-300
Mouse plasma	100-300
Rabbit serum	100-300
10% Rat liver tissue homogenate	50-150
10% Mouse kidney tissue homogenate	50-200
10% Rat brain tissue homogenate	200-400
10% Rat spleen tissue homogenate	50-200
Jukat cells	20-50

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.