What is the function of PMSF and Na3VO4 in Lysis buffer? And what is the concentration of this product and how to use it?

Both of these are enzyme inhibitors and their function is to prevent protein degradation. PMSF is often used as a protease inhibitor in biochemical and molecular biology experiments. It is an inhibitor of serine proteases and can inhibit the activities of trypsin, chymotrypsin and AChE. Na3VO4 is a phosphatase inhibitor, inhibiting ATPase, alkaline phosphatase and tyrosine phosphatase. The two products in the kit are 100X and should be added to the PIPA lysis buffer at a ratio of 1:100 before use.