Toll-free:1-888-852-8623

All categories

  • All categories
  • Flow Cytometry Antibodies
  • ELISA Kits
  • MACS Cell Isolation
  • Antibodies and Reagents
  • Cell Health Detection
  • Metabolism Assays
  • Immunoassays
  • Cell Identification
  • Proteins and Peptides
  • Cell Culture
Please enter the item number/product keyword!
Keyword cannot be empty !
INSERT SYMBOLS:
  • α
  • β
  • γ
  • δ
  • ε
  • ζ
  • η
  • θ
  • κ
  • μ
  • ω
  • σ
  • τ
  • λ
  • ⅩⅢ
  • ⅩⅢ
  • ⅩⅣ
  • ⅩⅤ
  • ⅩⅦ
  • ⅩⅧ
  • UP ↑

What is the function of PMSF and Na3VO4 in Lysis buffer? And what is the concentration of this product and how to use it?

Source: Elabscience®Published: Jul 01,2024

Both of these are enzyme inhibitors and their function is to prevent protein degradation. PMSF is often used as a protease inhibitor in biochemical and molecular biology experiments. It is an inhibitor of serine proteases and can inhibit the activities of trypsin, chymotrypsin and AChE. Na3VO4 is a phosphatase inhibitor, inhibiting ATPase, alkaline phosphatase and tyrosine phosphatase. The two products in the kit are 100X and should be added to the PIPA lysis buffer at a ratio of 1:100 before use.