Antibody & Protein
What is the function of PMSF and Na3VO4 in Lysis buffer? And what is the concentration of this product and how to use it?
Source: Elabscience®Published: Jul 01,2024
Both of these are enzyme inhibitors and their function is to prevent protein degradation.
PMSF is often used as a protease inhibitor in biochemical and molecular biology experiments. It is an inhibitor of serine proteases and can inhibit the activities of trypsin, chymotrypsin and AChE.
Na3VO4 is a phosphatase inhibitor, inhibiting ATPase, alkaline phosphatase and tyrosine phosphatase.
The two products in the kit are 100X and should be added to the PIPA lysis buffer at a ratio of 1:100 before use.