Cell Function FAQs

ElabFluor®488 offers better quenching resistance and is more suitable for longer microscopic observations than that of FITC.

Color development of DAB (result of TUNEL-HRP) is observed with an ordinary microscope, the results of TUNEL nuclear staining were served with white light. As for TUNEL fluorescence, the results of TUNEL and nuclear staining were observed with different fluorescence channels seperately with better definition and resolution.

1. To avoid the effect of light on TdT enzyme activity.
2. Biotin molecules are easily decomposed by light, and the double bonds in biotin molecules are easily excited by light, and the decomposition produces substances such as ultraviolet light and active free radicals. These decomposition products will destroy the chemical binding between biotin and labeled molecules, thus affecting the accuracy and stability of experimental results.

Yes they do. The blocking agent can be 3% H2O2. Peroxidase is present in many tissues, resulting in false positive results. Thus the endogenous peroxidase should be eliminated before staining. 3% hydrogen peroxide can react with peroxidase to eliminate endogenous peroxidase as a blocking agent.

There is no species limination in TUNEL assay kits.

Yes, it can be used to test sperm samples.

There is no species limination in TUNEL assay kits, so it will be avaliable theromatically. It is recommanded for customers to explore the method of sample preparation as there is no such data for insect samples.

The detection of TUNEL is based on DNA fragmentation in late stage apoptosis, so it is not avaliable for erythrocyte as there is no nuclei or other organelles containing DNA in erythrocyte.

(1) The slides can be sealed with anti-fluorescence quenching agent, but the microplate cannot.
(2) The amount of reagents will be increased with 6-well plate.
(3) The visual field will be decreased due to the smaller diameter of wells.

It is avaliable therometically, but it is recommanded to take a pre-test as there is no such data yet. You might need to notice some key points: 1. Protease K for section sample treatment as permeability agent may interfere the antigen of immunofluorescence; 2. High temperature or high pressure for antigen repair in immunofluorescence assay may lead to DNA fragmentation, that will end up with false positive TUNEL results.

Yes, the cells can be collected into EP tubes as cell suspension. After being stained by following the procedure, the cells can be detected by flow cyrometer, or the suspension can be sucked onto a slide and observed with a microscope.