TSA Multiplex Fluorescence Staining Kits

Multiplex immunohistochemistry (mIHC) with tyramide signal amplification (TSA) enables highly sensitive detection and spatial mapping of multiple biomarkers within a single tissue section.

Elabscience^® offers ready-to-use TSA Multiplex Fluorescence Staining Kits and flexible tyramide fluorophore combinations for custom multiplex panel design.

View All TSA Multiplex Fluorescence Staining Kits

Why Choose mIHC?

mIHC delivers higher resolution, enhanced specificity, and stable signals compared to conventional IF or IHC. Sequential staining with antibody stripping enables multiple rounds of detection while preserving the tissue’s spatial context, making mIHC ideal for analyzing the tumor microenvironment, immune infiltration, and complex signaling pathways.

Features

Co-localization of multiple biomarkers

High sensitivity and specificity

Compatible with primary antibodies from any species

Preserves spatial context

Applications

Tumor Microenvironment

Visualize spatial relationships and interactions among tumor cells, immune cells, and functional molecules.

Immune Microenvironment

Characterize immune cell subsets and assess activation, exhaustion, or immunosuppressive states.

Cellular Functions

Monitor key biological processes including senescence, apoptosis, ferroptosis, and autophagy.

Signaling Pathways

Analyze multiple pathways and protein interactions to uncover complex regulatory networks.

Product Portfolio

TSA Multiplex Fluorescence Staining Kits

Elabscience^® Multiplex Fluorescence Staining Kits deliver a complete, ready-to-use workflow for tissue multiplexing.

Product Name	Cat. No.	TSA Fluorophores	Nuclear Stain	Plex Format
TSA Multiplex Fluorescence Staining Kit (mIHC, 4 Plex)	E-IR-R333	TSA-520/TSA-570 /TSA-690 Fluorophores	DAPI	3 markers / 4 colors
TSA Multiplex Fluorescence Staining Kit (mIHC, 5 Plex)	E-IR-R330	TSA-520 /TSA-570 /TSA-620/TSA-690 Fluorophores	DAPI	4 markers / 5 colors
TSA Multiplex Fluorescence Staining Kit (mIHC, 6 Plex)	E-IR-R334	TSA-520/TSA-570/TSA-620/TSA-690/TSA-780 Fluorophores	DAPI	5 markers / 6 colors
TSA Multiplex Fluorescence Staining Kit (mIHC, 7 Plex)	E-IR-R335	TSA-480/TSA-520/TSA-570/TSA-620/TSA-690 /TSA-780 Fluorophores	DAPI	6 markers / 7 colors

Note: DAPI nuclear staining enables single-cell segmentation and spatial localization analysis.

Flexible Fluorophore Options

Elabscience^® multiple TSA fluorophores for flexible custom panel design.

TSA Fluorophores	Emission (nm)	Color
TSA-420 Fluorophore	420
TSA-440 Fluorophore	440
TSA-480 Fluorophore	480
TSA-520 Fluorophore	520
TSA-570 Fluorophore	570
TSA-600 Fluorophore	600
TSA-620 Fluorophore	620
TSA-640 Fluorophore	640
TSA-650 Fluorophore	650
TSA-670 Fluorophore	670
TSA-690 Fluorophore	690
TSA-770 Fluorophore	770
TSA-780 Fluorophore	780