Is it able to measure red fluorescence only when operating JC-1 assay?

JC-1 exists in monomer and polymer form, and their emission spectra are different. In normal cells with high mitochondrial membrane potentia, JC-1 accumulates in the matrix of mitochondria as polymer, which producing red fluorescence. Since the decreasing of mitochondrial membrane potential during early stage of apoptosis, JC-1 can no longer be accumulated in the mitochondrial matrix, that becomes monomer with green fluorescence. Therefore, both red and green fluorescence need to be observed.