Flow Cytometry Experimental Control Groups Setting - Single Positive Group

In this session, we're diving into the crucial role of Single Positive Control Tubes in multicolor flow cytometry experiments.

In multicolor flow cytometry, different fluorochromes can emit overlapping wavelengths, complicating data analysis. This is where single positive tubes come into play, helping to adjust and compensate for these overlaps.

In this video, we'll cover:

● What a single positive control tube is and why it's essential for your experiments.

● Key requirements for setting up single positive control tubes, including matching sample types, treatment consistency, and maintaining instrument voltage.

● The importance of having enough positive cells in your control tubes for accurate analysis.

● When you can skip single positive controls, particularly if your target marker is highly expressed.

● Tips for dealing with low-expression markers, including substituting them with high-expression markers like CD3 or CD4.

● Best practices for choosing fluorochromes to minimize spectral overlap and ensuring compensation values are within a reasonable range.

By the end of this video, you'll understand how to effectively use single positive control tubes to enhance the accuracy and reliability of your flow cytometry results!