Purified Flow Cytometry Antibodies for MSCs with Marker Selection, Validation Strategies, and Data-Driven Choices

Mesenchymal Stem Cells (MSCs) represent a cornerstone of the stem cell family-pluripotent cells originally isolated from bone marrow, with extraordinary capacity for MSC differentiation into osteogenic, chondrogenic, adipogenic and other tissue-forming lineages. Combined with their low immunogenicity, natural tropism to damaged tissues, and powerful immunomodulatory functions, MSCs are widely hailed as the "universal repair cells" and key immunoregulators driving modern regenerative medicine.

As a transformative approach in advanced cell therapy, mesenchymal stem cells therapy has become one of the most active and promising fields in translational medicine. Globally, more than 1,200 MSC-based clinical trials are actively underway, with over 27 commercial MSC products already approved for clinical use. These milestones collectively confirm the immense clinical potential, market value, and broad application prospects of MSCs, positioning them as a leading force in the future of regenerative and cell-based medicine.

Table of Contents

1. Flow cytometry markers for mesenchymal stem cells

2. Purified flow cytometry antibodies for human mesenchymal stem cells

3. How to validate purified flow cytometry antibodies for MSCs?

4. Where to buy purified flow cytometry antibodies for human MSCs with validation data?

5. Top-rated purified flow cytometry antibodies for mesenchymal stem cell research from Elabscience

01 Flow cytometry markers for mesenchymal stem cells

MSCs can be isolated and obtained from a variety of tissues, including bone marrow, umbilical cord, adipose tissue, dental tissue, and placenta. MSCs from different sources exhibit significant differences in cell abundance, proliferative capacity and functional properties^[1].

Table 1. Characteristics of MSCs from Different Tissue Sources

The Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy proposes minimal criteria to define human MSC.

MSC must be plastic-adherent when maintained in standard culture conditions.

MSC must express CD105, CD73 and CD90, and lack expression of CD45, CD34, CD14 or CD11b, CD79a or CD19 and HLA-DR surface molecules.

MSC must differentiate into osteoblasts, adipocytes and chondroblasts in vitro^[2].

02 Purified flow cytometry antibodies for human mesenchymal stem cells

Purified flow cytometry antibodies are essential for minimizing experimental interference and ensuring consistent, reproducible results in MSCs research. Elabscience's affinity purified antibodies for human MSCs are manufactured with advanced purification technologies and strict quality control, delivering unparalleled advantages:

High Specificity: Prepared from high-quality monoclonal antibodies with optimized clones, avoiding non-specific binding caused by impure components.

Fig. 1 High specificity Validation: Purified Anti-Human CD19 Antibody (Cat. No. E-AB-F10040P, Clone CB19).

High Stability: The antibody was stored at 37℃for 1,3,7 days, maintaining a highly consistent signal, confirming excellent product stability.

Fig. 2 High stability: Purified Anti-Human CD11b Antibody (Cat. No. E-AB-F1081A, Clone M1/70).

Low Background Interference: Optimized antibody selection and purification to minimize non-specific binding, enabling detection of rare cell subsets.

Fig. 3 Low Background Interference: Purified Anti-Human HLA-DR Antibody (Cat. No. E-AB-F1111A, Clone L243).

Comprehensive Coverage: Fully matches ISCT-recommended markers and additional functional markers, meeting diverse research needs from MSCs identification to subpopulation analysis.

Table 2. Purified Antibodies for Human MSCs Identification Markers

03 How to validate purified flow cytometry antibodies for MSCs?

Validating purified flow cytometry antibodies is critical to ensuring data credibility-poorly validated antibodies can lead to misleading results and research waste. Follow these practical strategies tailored for MSCs research:

3.1 Isotype control validation

Use corresponding isotype control antibodies to eliminate non-specific binding caused by the antibody Fc segment, ensuring accurate gating of positive cells.

3.2 Cell population validation

Test antibodies on both MSCs (positive for CD73 CD90 CD105) and non-MSCs cells (e.g., hematopoietic cells positive for CD45) to confirm specific binding to target markers.

3.3 Differentiation status validation

Verify antibody performance on both undifferentiated MSCs and differentiated MSCs (e.g., osteocytes), as marker expression (e.g., CD105, CD90) decreases after differentiation.

04 Where to buy purified flow cytometry antibodies for human MSCs with validation data?

For researchers seeking reliable purified flow cytometry antibodies for human MSCs with complete validation data, Elabscience^® is your one-stop solution-combining high-quality products, transparent validation, and convenient global procurement:

• Complete validation data: Every batch of antibodies comes with detailed validation reports, fully supporting data-driven research choices.

• Global procurement channels: Order directly via Elabscience^® official website (www.elabscience.com), email (orders@elabscience.com), or phone (1-888-852-8623).

• Flexible support: Custom antibody services, bulk purchase discounts, and 24-hour technical consultation(techsupport@elabscience.com) are available to meet diverse research needs.

05 Top-rated purified flow cytometry antibodies for mesenchymal stem cell research from Elabscience^®

Elabscience provides top-rated purified flow cytometry antibodies for MSCs research, all experimentally validated for MSCs applications. Below are Elabscience's best-sellers for human MSCs identification:

CD73、CD90、CD105: Validated on Umbilical cord mesenchymal stem cells, ensuring > 95% positive detection rate for MSCs identity verification.

CD45、CD11b、CD19、CD34、HLA-DR: Validated as MSCs negative control, effectively excluding hematopoietic contamination (< 2% positive rate).

All antibodies undergo strict in-house QC, ensuring consistent, reliable performance backed by experimental validation.

Accurate MSCs analysis relies on validated antibodies. Elabscience delivers experimentally proven purified flow cytometry antibodies for MSCs research, supporting global researchers with cost-effective, reliable solutions.

Fig. 4 Flow Cytometry Detection Results of Surface Markers for Human Umbilical Cord Mesenchymal Stem Cells.

References:

[1] Chen Q, Shou P, Zheng C, et al. Fate decision of mesenchymal stem cells: adipocytes or osteoblasts?. Cell Death & Differentiation. 2016; 23 (7): 1128-1139.

[2] M Dominici 1, K Le Blanc. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315-7.