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Maltase Activity Assay Kit

Cat:E-BC-K041-M
Manual MSDS

Price: $ 200

Size:
96T
Quantity:
  • Sample Type: animal tissue
  • Detection Instrument: Microplate reader(500 nm-520 nm,optimum wavelength: 505 nm)
  • Reasearch Areas: Glycolysis Lipid Metabolism
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For research use only. Order now, ship in 3 days

Product Details
Detection Principle Maltase catalyze the corresponding substrate to produce monosaccharide. Monosaccharide produce hydrogen peroxide under the action of oxidase. Hydrogen peroxide react with chromogenic agent to form red product. The activity of maltase can be calculated by detection the optical density with spectrophotometer at 505 nm.
Sample Type animal tissue
Sensitivity 6.32 U/mL
Detection Range 6.32-750 U/mL
Detection Method Colorimetric method
Assay Time 60 min
Precision inter-assay CV: 5.7% | intra-assay CV: 2.7%
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
10% Rat heart tissue homogenate 1
10% Rat liver tissue homogenate 1
10% Rat spleen tissue homogenate 1
10% Mouse intestinal tissue homogenate 1
10% Mouse kidney tissue homogenate 1
10% Mouse brain tissue homogenate 1
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.