Myeloperoxidase (MPO) Activity Assay Kit (E-BC-K074-S)
                        For research use only.
| Detection Principle | Myeloperoxidase reduces hydrogen peroxide to a complex. The complex react with o-dianisidine (as hydrogen donor) to produce a yellow product which has a maximum absorption peak at 460 nm. The activity of MPO can be calculated indirectly by measuring the OD value at 460nm. | 
| Synonyms | MPO | 
| Sample Type | Serum,Plasma,Milk,Animal tissue,Cell | 
| Detection Method | Colorimetric method | 
| Detection Instrument | Spectrophotometer (460 nm) | 
| Research Area | Oxidative Stress | 
| Storage | This product can be stored at 2-8°C for 12 months with shading light. | 
| Valid Period | 12 months | 
| Sensitivity | 16.95 U/L | 
| Detection Range | 16.95-3349 U/L | 
| Precision | inter-assay CV: 9.8% | intra-assay CV: 4.5% | 
| Sample Volume | 450-900 μL | 
| Assay Time | 1 h 30 min | 
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor | 
|---|---|
| Human serum | 1 | 
| Human plasma | 1 | 
| Rat serum | 1 | 
| 5% Mouse brain tissue homogenization | 1 | 
| 5% Mouse heart tissue homogenization | 1 | 
| Human milk | 1 | 
| Human serum | 1 | 
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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