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Cat.No.:E-BC-K074-M
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Synonyms | MPO |
Sample type | Serum, plasma, animal tissue |
Sensitivity | 19.42 U/L |
Detection range | 19.42-893.31 U/L |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 65 min |
Precision | Average inter-assay CV: 7.300%Average intra-assay CV: 5.400% |
Other instruments required | Tubes, Micropipette, Vortex mixer, 37℃/60℃ Water bath |
Storage | 2-8℃ |
Valid period | 12 months |
The activity of MPO indicating the neutrophil level was slightly higher in the KO (C3 knockout) than WT (wild type) mice.
Y J Choi investigate the effective of complement component 3 (C3) deficiency promoting its receptor downstream-mediated inflammatory response. Myeloperoxidase (MPO) activity of mouse colon was determined using MPO activity assay kit (E-BC-K074-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (19.42-893.31 U/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Human plasma |
1 |
Human milk |
1 |
Cell culture supernatant |
1 |
Rat serum |
1 |
Rat plasma |
1 |
5% Rat kidney tissue homogenate |
1 |
5% Rat spleen tissue homogenate |
1 |
Note: The diluent is reagent 2 application solution.
Sample: colon tissue |
Sample: neutrophils |
DOI: 10.1155/2022/8217663 |
|
PMID: 35178161 |
Sample: plasma |
Sample: serum |
Sample: testicular tissue |
DOI: 10.1002/tox.23639 |
|
Sample: serum,NR8383 cell |
Sample: colon tissue |
Sample: colon |
PMID: 35105928 |
Sample: colon |
PMID: 35259712 |
Sample: stomach tissues |
DOI: 10.1155/2022/6205190 |
|
Sample: H9C2 cell |
DOI: 10.1002/jbt.23240 |
|
Sample: blood |
DOI: 10.1002/iid3.466 |
|
PMID: 34102007 |
Sample: plasma |
Sample: neutrophils |
DOI: 10.1002/jez.2603 |
|
Sample: brain |
M*****iSubmitted [ Oct 13 2022 ]
Asked: For tissue samples, can I calculate by protein content?
Reply
adminSubmitted [ Oct 13 2022 ]
Answered: NO, because the homogenate used in this kit has an effect on protein detection.
M*****iSubmitted [ Sep 06 2022 ]
Asked: Can I use PBS instead of reagent 1 as the homogenate?
Reply
adminSubmitted [ Sep 06 2022 ]
Answered: No. The homogenate can not be replaced by PBS, otherwise the sample will be extracted insufficiently, resulting in low results.