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Myeloperoxidase (MPO) Peroxidation Activity Fluorometric Assay Kit

Cat.No.:E-BC-F013
Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

Detection method: Fluorescence method

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96T

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Detection principle

Under the catalysis of peroxidase, hydrogen peroxide can oxidize the non-fluorescent probe into the fluorescent substance, and its fluorescence intensity is proportional to the total peroxidase activity in the sample. This kit specifically inhibits the peroxidase activity of MPO in the sample through an MPO enzyme inhibitor, thus distinguishing the peroxidase activity of MPO in the sample from that of other peroxidases.

Performance characteristics

Synonyms	MPO
Sample type	Serum, plasma, animal tissue
Sensitivity	0.001 U/L
Detection range	0.001 - 1.26 U/L
Detection method	Fluorescence method
Assay type	Enzyme Activity
Assay time	20 min
Precision	Average inter-assay CV: 5.400%Average intra-assay CV: 1%
Other instruments required	Micropipette, Vortex mixer, Water bath
Storage	-20℃
Valid period	12 months

Images

MPO activity was significantly increased in DSS model, and decreased with cinacalcet treated. (*P<0.05, **P<0.01, ***P<0.001)

Y Chen et al investigate the protective effect of cinacalcet against colitis induced by dextran sulfate sodium. Myeloperoxidase (MPO) peroxidation activity of mouse colon tissue was determined using MPO activity fluorometric assay kit (E-BC-F013).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.001 - 1.26 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Porcine serum	5-10
Rabbit serum	3-5
Rat serum	2-5
Mouse serum	10-20
Mouse plasma	30-50
Horse serum	2-5
10% Rat heart tissue homogenate	1
10% Rat lung tissue homogenate	1

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714
Fexofenadine protects against lipopolysaccharide-induced acute lung injury by targeting cytosolic phospholipase A2

DOI: 10.1016/j.intimp.2022.109637

Sample: lung tissue

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