Sodium (Na+) Colorimetric Assay Kit (E-BC-K207-S)
For research use only.
| Detection Principle | The formation of nitrophenol from the substrate nitropyranoside is catalyzed by sodium-activated β-galactosidase. The increase rate of absorbance value of nitrophenol at 405 nm per unit time was proportional to the sodium concentration. |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (405 nm) |
| Research Area | Inorganic Ions , Oxidative Stress |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.02 mmol/L |
| Detection Range | 0.02-10 mmol/L |
| Precision | inter-assay CV: 7.5-8.9% | intra-assay CV: 2.3-4.5% |
| Sample Volume | 50 μL |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 15-25 |
| Mouse serum | 15-25 |
| Human plasma | 15-25 |
| Rat plasma | 15-25 |
| 10% Mouse liver tissue homogenate | 15-25 |
| 10% Rat heart tissue homogenate | 15-25 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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