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Sucrase Activity Assay Kit

Cat.No.:E-BC-K751-M
Detection instrument: Microplate reader (500-520 nm, optimum wavelength: 505 nm)

Detection method: Colorimetric method

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96T

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Detection principle

Sucrase catalyzes its substrate (sucrose) to produce glucose, which produces hydrogen peroxide under the action of glucose oxidase. Hydrogen peroxide reacts with chromogenic agent to produce red substance, which has a strong absorption peak at 505 nm. In a certain concentration range, It's absorbance is proportional to glucose concentration. Therefore, the activity of sucrase can be calculated by measuring the OD value at 505 nm.

Performance characteristics

Sample type	animal tissu
Sensitivity	20 U/mL
Detection range	20-2000 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	70 min
Precision	Average inter-assay CV: 6.500%Average intra-assay CV: 5.400%
Other instruments required	Micropipette, Vortex mixer, Centrifuge
Other reagents required	PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (20-2000 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
20% Rat ileum tissue homogenate	1
20% Rat stomach tissue homogenate	1
20% Rat liver tissue homogenate	1

Note: The diluent is PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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