Sucrase Activity Assay Kit (E-BC-K751-M)

Name: Sucrase Activity Assay Kit
Brand: Elabscience
SKU: E-BC-K751-M
Price: 320.0 USD
Availability: InStock

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	96T	$ 320.00	- +

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Detection Principle	Sucrase catalyzes its substrate (sucrose) to produce glucose, which produces hydrogen peroxide under the action of glucose oxidase. Hydrogen peroxide reacts with chromogenic agent to produce red substance, which has a strong absorption peak at 505 nm. In a certain concentration range, It's absorbance is proportional to glucose concentration. Therefore, the activity of sucrase can be calculated by measuring the OD value at 505 nm.
Sample Type	Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (500-520 nm, optimum wavelength: 505 nm)
Research Area	Plant Physiology Series
Other Reagents Required	PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	20 U/mL
Detection Range	20-2000 U/mL
Precision	inter-assay CV: 6.5% \| intra-assay CV: 5.4%
Sample Volume	50 μL(tissue homogenate)
Assay Time	1 h 10 min

Cat.No.	Product Name	Sizes
E-BC-F042	Sucrose Fluorometric Assay Kit	96T
E-BC-K161-S	Sucrose Colorimetric Assay Kit	100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
20% Rat ileum tissue homogenate	1
20% Rat stomach tissue homogenate	1
20% Rat liver tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.