Current Position:Home>>Kits>>Metabolism Assays>> Sucrose Colorimetric Assay Kit

Sucrose Colorimetric Assay Kit

Cat.No.:E-BC-K161-S
Detection instrument: Spectrophotometer(290 nm)

Detection method: Colorimetric method

To Purchase E-BC-K161-S

Size:

100Assays
50Assays

Price: $260

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose.

Performance characteristics

Sample type	Plant tissue
Sensitivity	0.32 μmol/mL
Detection range	0.32-70 μmol/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	40 min
Precision	Average inter-assay CV: 8.500%Average intra-assay CV: 3.400%
Other instruments required	Micropipettor, Vortex mixer, 100℃ Water bath
Other reagents required	PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.32-70 μmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Green pepper tissue homogenization	1
10% Epipremnum aureum tissue homogenization	1
10% Cucumber tissue homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

Cat. No	E-BC-K161-S
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha