Sucrose Colorimetric Assay Kit (E-BC-K161-S)
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For research use only.
| Detection Principle | Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (290 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.32 μmol/mL |
| Detection Range | 0.32-70 μmol/mL |
| Precision | inter-assay CV: 8.5% | intra-assay CV: 3.4% |
| Sample Volume | 30 μL(tissue homogenate) |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Green pepper tissue homogenization | 1 |
| 10% Epipremnum aureum tissue homogenization | 1 |
| 10% Cucumber tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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