(KO Validated) HuR / ELAVL1 Polyclonal Antibody
- +5
Price: $ 580
Price: $ 360
Price: $ 220
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB;IHC;IF
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:U-251MG,293T,HeLa,Jurkat,K562,PC-3 Verified Samples in IHC:Mouse testis,Mouse kidney,Rat testis,Human kidney cancer,Human esophageal cancer Verified Samples in IF:MCF-7 |
Dilution |
WB 1:500-1:2000, IHC 1:100-1:200, IF 1:50-1:200 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Recombinant fusion protein of human HuR / ELAVL1 (NP_001410.2). |
Abbre | HuR / ELAVL1 |
Synonyms | ELAV1;HUR;Hua;MelG;HuR / ELAVL1;ELAVL1 |
Swissprot | |
Calculated MW | 36kDa/38kDa |
Observed MW |
37kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cytoplasm. |
Concentration | 1mg/mL |
Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method | Affinity purification |
Research Areas | Cancer; Epigenetics and Nuclear Signaling |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. |